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Abstract: The abstract should summarize your suggestion. Add a sentence to introduce the problem that examine why this problem is important why the hypothesis to be tested is a brief summary of the experiments you want to perform, and a single final sentence. (500 word limit)
Introduction: The introduction discusses the background and meaning of the problem you examine. Lead the reader from the general to specific. For example, if you want to write about the role that BRCA1 mutations play in breast cancer pathogenesis, they first talk about the importance of breast cancer as a disease in the US / world population, then about family breast cancer as a small subset of breast cancer generally over the discovery from BRCA1 mutations in family breast cancer, then the normal functions of BRCA1 in the DNA repair, then how Brca1 mutations lead to damaged DNA and the beginning of familiar breast cancer, etc., definitely figures with properly labeled text fields (designated) like illustration 1, Figure 2, etc.) Here, to better illustrate your points and help your reader to wade through unknown science.
hypothesis: Formulate a hypothesis that is tested in your subsidy proposal. Remember, they make a hypothesis-powered research so that a hypothesis should be tested! The hypothesis should be focused from the introduction, concise and flow. For example, your hypothesis could be "I hypothesize that the overexpressing wild type BRCA1 will prevent Metastatic Spreiz in a mouse Xenogo model in BRCA1 zero tumor cells." Based on your hypothesis, your specific target section should be aligned to support it. The hypothesis is given in a set in the proposal.
Special goals (listed as specific destination 1, specific objective 2): You want to work together with your mentor to make the experimental part of your proposal. Beat two original specific goals to test your hypothesis. Do not suggest more than two goals - they do not have enough time to do more. In the illustrated example, a specific target 1 may be "to determine the oncogenic potential of BRCA1 zero cell lines expressing the wild type BRCA1 cDNA". The special goal 2 could be "to determine the metastatic potential of BRCA1 zero cells expressing WT BRCA1. You do not have to go into extensive technical details, only for the reader to understand what you suggest. The best goals Mechanistic insights - that is, experiments suggested to tackle some mechanisms of biology. A less desirable goal proposes correlative experiments that mechanically do not address how BrCA1 mutations produce cancer. It is also very important that the two goals are coherent but are not dependent on each other. What if AIM 1 does not work, AIM 2 is not automatically dead. Tell, for example, that you suggest in AIM 1 to generate a BRCA1 knockout mouse model, and in AIM 2 Take tissue from this mouse to do experiments. If you knock BRCA1 to an early embryonic death, you will never be a ma Us get the cloths supplies for AIM. 2. Here you can include some of the data of your mentor as "preliminary data". Remember to carefully cite all your sources.
potential pitfalls and alternative strategies: This is a very important part of a proposal. Here you would like to discuss the experiments suggested by you 1 and 2. Remember that no experiment is perfect. Are there any reasons why experiments from which they suggested do not work? Why? What will you do to fix this? What are other potential strategies that you use if your experiments do not work? If a revenge reveals these deficiencies and suggest no methods to correct them, their proposal will not be financed. You want to work with your mentor to write this section.
References: Quote all references, including unpublished data from your mentor. Last, first, (year), title, journal, volume, pages.
* 8-page default boundary (not including references), 1.5 distance, 12PT times new Roman font
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